上海浩洋生物科技有限公司

 HYALURONIDASE, S. HYALURONLYTICUS NOV
  • HYALURONIDASE, S. HYALURONLYTICUS NOV
  • HYALURONIDASE, S. HYALURONLYTICUS NOV

    参考价格: ¥1471.00
    品  牌:merck
    产品型号:389561-100U (100U)
    所在地区:上海
    上架时间:2013年04月27日
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    HYALURONIDASE, S. HYALURONLYTICUS NOV
    产品名称:HYALURONIDASE, S. HYALURONLYTICUS NOV
    产品货号:389561-100U
    产品价格:¥1471
    产品规格:100U
    Application Notes: Assay for Enzymatic Activity
    Reagent Preparation
    • Prepare acetate buffer: 150 mM NaCl, 20 mM sodium acetate-acetic acid buffer, pH 6.0.
    • Prepare hyaluronic acid solution: 50 mg purified potassium hyaluronate (containing approximately 38% glucuronic acid) is dissolved in 100 ml of the above mentioned sodium acetate buffer.
    • Measure acidic bovine serum albumin with a spectrophotometer at 280 nm. Adjust the total absorbance of the albumin solution to 910. Dilute to 100 ml with 0.5 N acetate buffer, pH 4.3. Adjust the pH of the albumin solution to 3.1 using 4 N HCl. Boil in a water bath for 30 min at 100°C. Adjust to 100 ml with distilled H2O. This solution is stable for 3-4 weeks at 4°C. To use this solution, dilute with 5 volumes of acetate buffer from above.
    Activity Protocol
    • For the assay, mix 0.5 ml of the enzyme solution (made with acetate buffer without NaCl) with 0.5 ml of the hyaluronic acid solution. Incubate for 30 min at 60°C.
    • Add 4 ml of the acidic bovine serum albumin solution. Incubate for 10 min at 60°C.
    • Measure the turbidity at 660 nm. Obtain a blank value by using deactivated enzyme (incubate at 100°C for 10 min). Correct the reading by subtracting the blank value.
    (blank value - measured value/blank value) x 2 x 2 x dilution = TRU/ml
    Molecular Weight: 91000
    Presentation: White lyophilized powder.
    Contaminants: Protease: ≤ 0.05 units/ampule (pH 6.0)
    Specific Activity: ≥2000 U/A280
    Properties: Solubility: 500 mM Acetate buffer, pH 6.0 (30 U/ml) or H2O (30 U/ml).
    Storage Conditions: Shipped at Ambient Temperature. Store at +2 - 8°C.