产品名称:Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight488 Conjugate
Description:Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight?488 Conjugate
Concentration:1.0 mg/ml (E 1% at 280 nm = 13.0)
Conjugate:DyLight? 488 (Ex = 493 nm; Em = 518 nm)
Purification:Affinity purified using solid phase Rabbit IgG
Purity:Affinity purified antibody is > 95% based on SDS-PAGE
Immunogen:Purified Rabbit IgG, whole molecule
Buffer:10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:0.05% (w/v) Sodium Azide
Reconstitution:Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:Store freeze-dried powder at 2-8 °C.
Shelf Life:Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:Based on IEP, this antibody reacts with:
?heavy (γ) chains on rabbit IgG
?light chains on all rabbit immunoglobulins
Cross Reactivity:Based on IEP, no reactivity is observed to:
?non-immunoglobulin rabbit serum proteins
Country of Origin:Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Applications:This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):
?1:20 - 1:2,000 for most applications