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 Human Granzyme B ELISPOT Ready-SET-Go
  • Human Granzyme B ELISPOT Ready-SET-Go
  • Human Granzyme B ELISPOT Ready-SET-Go

    参考价格: ¥7500.00
    品  牌:eBioscience
    产品型号:88-8399-88 (10 plates)
    所在地区:上海
    上架时间:2013年01月21日
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    Human Granzyme B ELISPOT Ready-SET-Go
    产品名称:Human Granzyme B ELISPOT Ready-SET-Go
    规格:10 plates
    货号:88-8399-88
    厂商:eBioscience
    产品介绍:
    Also known as: GrzB, GrB
    Reactivity Human
    Components
    Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody
    Detection Antibody. Pre-titrated, biotin-conjugated antibody
    ELISA/ELISPOT Coating Buffer. This Ready-Set-Go! ELISPOT Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 uM).
    Assay Diluent. 5X concentrated
    Detection enzyme. Pre-titrated Avidin-HRP
    Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
    Reported Applications ELISPOT
    Description: One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell. The granules contain a number of proteins, including the pore-forming protein perforin and a family of serine proteases called granzymes, including Granzyme B. Granzyme B is present mainly in the granules of CD8+ CTL and natural killer (NK) cells and mediates the lethal hit that kills virus infected and tumorigenic cells. Measurement of release of Granzyme B in response to the appropriate target is useful for evaluating cell-mediated cytotoxicity. The Granzyme B ELISPOT assay is a valuable non-radioactive alternative to the 51Cr-release killing cell assays for measuring antigen-specific CTL cytotoxicity.
    eBioscience 88-8399-88 Human Granzyme B ELISPOT Ready-SET-Go! Set 10 plates 7500
    eBioscience 88-8399-21 Human Granzyme B ELISPOT Ready-SET-Go! Set 2 plates 3000