产品名称：Big Endothelin-1 (human), ELISA kit
规格：1 x 96 wells
Long Term Storage: +4°C
Sensitivity: 0.23 pg/ml (range 0.78 - 100 pg/ml)
Application: For the quantitative determination of human Big ET-1 in culture supernatants and serum. Cited species and sample type include rabbit and lung lavage fluid (ref. 1).
Kit/Set Contains: Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
Miscellaneous/General: Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.