产品名称：hROS, (detection reagent)
ong Term Storage: +4°C
Name: Reactive Oxygen Species
Formulation: Liquid. APF (aminophenyl fluorescein) in DMF. Sufficient for ~150 tests.
Application: Detect and quantitate hROS via Flow Cytometry cytometry, immunofluorescent microscopy, or fluorescent plate-based assay
Miscellaneous/General: The hROS Detection Reagent, Aminophenyl fluorescein (APF), is a selective indicator for highly reactive oxygen species (hROS). APF is a cell permeable dye that can be used to detect hydroxyradical, peroxynitrite, and hypochlorite production in cells. This fluorescent reagent has little reactivity towards other ROS such as singlet oxygen, superoxide, hydrogen peroxide, nitric oxide, and alkyl peroxide. APF itself is not very fluorescent, however when reacted with hROS strong dose dependent fluorescence is exhibited. This versatile detection reagent can be adapted for use in quick one-step homogenous high throughput fluorescent plate-based assays, immunofluorescent microscopy, or flow cytometry.