Long Term Storage: +4°C
Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human ASK1. The sequence differs from mouse ASK1 by two amino acids.
Application: Immunocytochemistry (10µg/ml)
Western Blot (1:500-1:1000)
Optimal conditions must be determined individually for each application.
Specificity: Recognizes human ASK1. Detects a band of ~155kDa by Western blot.
Purity Detail: Peptide affinity purified.
Formulation: Liquid. In PBS containing 0.02% sodium azide.
Miscellaneous/General: Apoptosis signal-regulating kinase 1 (ASK1), also known as MAPKKK5 is a member of the MAPKKK family. ASK1 activates two different subgroups of MAPKK, MKK4, and MKK6, which in turn activate c-Jun N-terminal kinase (Jnk) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNF-R and Fas through the interaction with members of the TRAF family and the Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF-alpha-induced apoptosis. ASK1 is expressed in a variety of human and mouse tissues.