Long Term Storage: -20°C
Immunogen: Synthetic peptide corresponding to a portion of mouse Fas.
Application: Immunohistochemistry (1:50)
Western Blot (1:1000, ECL)
Optimal conditions must be determined individually for each application.
Specificity: Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
Purity Detail: Peptide affinity purified.
Formulation: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Miscellaneous/General: Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Both Fas and TNFR contain a 70 amino acid cytoplasmic “death domain” responsible for transmitting signals for apoptosis. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells.
ENZO ADI-AAP-221-D Fas, pAb 50 ug 2700
ENZO ADI-AAP-221-F Fas, pAb 200 ug 5250