产品名称：β-Catenin, mAb (12F7)
Alternative Name: CTNNB, cadherin-associated protein
Long Term Storage: -20°C
Immunogen: Recombinant β-catenin (N-terminal fragment).
Application: Immunohistochemistry (1-5µg/ml)
Immunoprecipitation (2µg/200-300µl cell extract)
Western Blot (1µg/ml, ECL)
Optimal conditions must be determined individually for each application.
Specificity: Recognizes human, mouse and rat β-catenin. Detects bands of ~97kDa by Western blot.
Purity Detail: Protein A affinity purified.
Formulation: Liquid. In PBS, pH 7.2, containing 50% glycerol.
Miscellaneous/General: β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus. This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway. Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation. β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote expression of a variety of target genes, including c-myc and cyclin D1.