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 β-Catenin, mAb (12F7)
  • β-Catenin, mAb (12F7)
  • β-Catenin, mAb (12F7)

    参考价格: ¥3600.00
    品  牌:ENZO
    产品型号:ADI-KAM-ST001-E (100 ug)
    所在地区:上海
    上架时间:2013年03月05日
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    β-Catenin, mAb (12F7)
    产品名称:β-Catenin, mAb (12F7)
    规格:100 ug
    货号:ADI-KAM-ST001-E
    厂商:ENZO
    产品介绍:
    Alternative Name: CTNNB, cadherin-associated protein
    Clone: 12F7
    Host: Mouse
    Isotype: IgG1
    Long Term Storage: -20°C 
    Immunogen: Recombinant β-catenin (N-terminal fragment).
    Application: Immunohistochemistry (1-5µg/ml)
    Immunoprecipitation (2µg/200-300µl cell extract)
    Western Blot (1µg/ml, ECL)
    Optimal conditions must be determined individually for each application.
    Specificity: Recognizes human, mouse and rat β-catenin. Detects bands of ~97kDa by Western blot.
    Purity Detail: Protein A affinity purified.
    Formulation: Liquid. In PBS, pH 7.2, containing 50% glycerol.
    Miscellaneous/General: β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus.  This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway.  Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation.  β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote  expression of a variety of target genes, including c-myc and cyclin D1.