产品名称：One-STrEP Starter Kit
The pEXPR-IBA103 plasmid is based on pEXPR-IBA3 but contains the new One-STrEP-tag (for C-terminal bait fusion) instead of Strep-tag® II. Junttila MR, Saarinen S, Schmidt T, Kast J, Westermarck J (2004) Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells. Proteomics, 5, 1199-1203, 2005.
Both PR65 and PP2Ac are subunits of the phosphatase 2A protein complex, which is present in most mammalian cells. A positive detection of the prey in the eluate proves the successful isolation of an intact protein complex [Junttila et al., 2005].