产品名称：PP2Ac affinity-purified goat polyclonal antibody
PPAc affinity-purified goat polyclonal antibody is a control antibody against PP2Ac (C-20) for use in studying protein:protein interactions. A mammalian control donor vector containing the coding sequence of PR65 is available (Cat. No. 2-1010-000) to generate an expression vector to express the PR65 bait in the cell line of choice (human cell lines like HT1080, 293 or HeLa are recommended). PR65 is a structural subunit of the PP2A phosphatase holoenzyme and thus should bind PP2Ac (36 kDa), the respective catalytic subunit, leading to its co-purification. Using this polyclonal antibody directed against PP2Ac, protein complex formation can thus be verified by immunodetection of PP2Ac by Western blotting.