上海浩洋生物科技有限公司

 StarGate Acceptor Vector pLSG-IBA123
  • StarGate Acceptor Vector pLSG-IBA123
  • StarGate Acceptor Vector pLSG-IBA123

    参考价格: ¥1248.00
    品  牌:IBA
    产品型号:5-4923-005 (5 rxns)
    所在地区:上海
    上架时间:2013年03月12日
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    StarGate Acceptor Vector pLSG-IBA123
    产品名称:StarGate Acceptor Vector pLSG-IBA123
    规格:5 rxns
    货号:5-4923-005
    厂商:IBA
    产品介绍:
    The pLSG-IBA123 vector allows the expression of Twin-Strep®/GST-tag-fusion-proteins in insect cells. The vector carries the Polyhedrin promoter for high-level expression in insect cells, the Twin-Strep-tag® for C-terminal and the GST-tag for N-terminal fusion to the recombinant protein. The Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in E.coli the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be localized in the cytoplasm. The additional PreScission protease cleavage site allows the removal of the GST-tag.
    IBA 5-4923-005 StarGate Acceptor Vector pLSG-IBA123 5 rxns 1248
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