Storage Condition -20 C
10X Reaction Buffer 670 mM glycine-KOH (pH 9.4), 25 mM MgCl2, 0.1% (v/v) Triton X-100
Concentration 10 U/µL
Definition of Activity Unit •One unit of the enzyme catalyzes the release of 10 nmol of acid soluble reaction products from double-stranded substrate in 30 min at 37ºC.
•Enzyme activity is assayed in the following mixture: 67 mM glycine-KOH (pH 9.4), 2.5 mM MgCl2, 0.1% (v/v) Triton X-100 and 20 µg/mL sonicated E. coli [3H]-DNA.
Inactivation Inactivated by heating at 80°C for 15 min.
Inhibition Inhibitors: salt at relatively low concentration (0.2 M KCl, 0.1 M NaCl), p-chloromercuribenzoate.
Molecular Weight 26 kDa monomer
Generating single-stranded PCR products for use in:
DNA sequencing (see? Reference 3)
Analysis of DNA single-strand conformation polymorphism (SSCP) (see? Reference 4)
Rolling circle amplification
Producing single-stranded DNA from double-stranded DNA fragments
Cloning of PCR products (see Reference 5)
MBI EN0561 Lambda Exonuclease 1000 units 577
MBI EN0562 Lambda Exonuclease 5000 units 2307