上海浩洋生物科技有限公司

 Lambda Exonuclease
  • Lambda Exonuclease
  • Lambda Exonuclease

    参考价格: ¥2307.0000
    品  牌:MBI
    产品型号:EN0562 (5000 units)
    所在地区:上海
    上架时间:2013/3/13 9:38:34
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    Lambda Exonuclease
    产品名称:Lambda Exonuclease
    规格:5000 units
    货号:EN0562
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    10X Reaction Buffer 670 mM glycine-KOH (pH 9.4), 25 mM MgCl2, 0.1% (v/v) Triton X-100
    Concentration 10 U/µL
    Definition of Activity Unit •One unit of the enzyme catalyzes the release of 10 nmol of acid soluble reaction products from double-stranded substrate in 30 min at 37ºC.
    •Enzyme activity is assayed in the following mixture: 67 mM glycine-KOH (pH 9.4), 2.5 mM MgCl2, 0.1% (v/v) Triton X-100 and 20 µg/mL sonicated E. coli [3H]-DNA.
    Inactivation Inactivated by heating at 80°C for 15 min.
    Inhibition Inhibitors: salt at relatively low concentration (0.2 M KCl, 0.1 M NaCl), p-chloromercuribenzoate.
    Molecular Weight 26 kDa monomer
    Applications
    Generating single-stranded PCR products for use in:
    DNA sequencing (see? Reference 3)
    Analysis of DNA single-strand conformation polymorphism (SSCP) (see? Reference 4)
    Rolling circle amplification
    Producing single-stranded DNA from double-stranded DNA fragments
    Cloning of PCR products (see Reference 5)
    MBI EN0561 Lambda Exonuclease 1000 units 577
    MBI EN0562 Lambda Exonuclease 5000 units 2307