Storage Condition -20 C
Formula C5H3FN2O4 x H2O
Molecular weight 192.1
Quality Control Purity of 98%.
Storage Store at -20°C in the dark.
The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Detection of URA3 gene expression in yeast
Construction of yeast 2-hybrid libraries (see Reference 1)
Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations (see? Reference 2)
Low solubility in water
Solubility in dimethylsulfoxide is greater than 150 mg/mL
Preparation of a 100 mg/mL solution in dime-thylsulfoxide is recommended