Storage Condition Ambient
1X Composition 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4
Quality Control The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.
Storage There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.
Usage Recommendations •Use fresh 1X TAE both for the gel and for the electrophoresis run.
To prepare 1X TAE buffer add 20 mL of 50X TAE buffer to 980 mL of deionized water and mix well.
Electrophoresis of nucleic acids in agarose and polyacrylamide gels
Used both as a running buffer and as a gel preparation buffer
Filtered through a 0.22 µm membrane
Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.
MBI ER1581 AarI 25 units 721
MBI ER1582 AarI 125 units 2884