产品名称：DreamTaq DNA Polymerase
Storage Condition -20 C
Definition of Activity Unit One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
Inactivation Inactivated by phenol/chloroform extraction
Inhibition Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
Robust amplification with minimal optimization
High yields of PCR products
Higher sensitivity compared to conventional Taq DNA polymerase
Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
Incorporates modified nucleotides, but does not incorporate dUTP
Routine PCR amplification of DNA fragments up to 6 kb
High throughput PCR
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl2.
MBI EP0703 DreamTaq™ DNA Polymerase 5x500 units 927
MBI EP0704 DreamTaq™ DNA Polymerase 20x500 units 3296