上海浩洋生物科技有限公司

 DreamTaq DNA Polymerase
  • DreamTaq DNA Polymerase
  • DreamTaq DNA Polymerase

    参考价格: ¥927.00
    品  牌:MBI
    产品型号:EP0703 (5x500 units)
    所在地区:上海
    上架时间:2013年03月13日
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    DreamTaq DNA Polymerase

    产品名称:DreamTaq DNA Polymerase
    规格:5x500 units
    货号:EP0703
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    Definition of Activity Unit One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
    Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
    Inactivation Inactivated by phenol/chloroform extraction
    Inhibition Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
    Highlights
    Robust amplification with minimal optimization
    High yields of PCR products
    Higher sensitivity compared to conventional Taq DNA polymerase
    Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
    Incorporates modified nucleotides, but does not incorporate dUTP
    Applications
    Routine PCR amplification of DNA fragments up to 6 kb
    High throughput PCR
    Genotyping
    Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl2.
    MBI EP0703 DreamTaq™ DNA Polymerase 5x500 units 927
    MBI EP0704 DreamTaq™ DNA Polymerase 20x500 units 3296