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EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

参考价格:¥1082.00
品  牌:MBI
产品型号:K1441 (200 react)
所在地区:华东 上海
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详细说明

EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
产品名称:EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
规格:200 react
货号:K1441
厂商:MBI
产品介绍:
Storage Condition -20 C
Hazardous No
10X EpiBuffer Composition 1 mg/mL BSA, 330 mM Tris-acetate (pH 7.9), 1 mM Acetic acid, 660 mM Potassium acetate
Quality Control Functional Testing
The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI.
Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes.
Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.
Highlights
Fast – complete digestion of genomic DNA in 1 hour at 37°C
Efficient – specially formulated enzymes for epigenetic studies of DNA methylation status
Applications
CpG methylation analysis at 5’-CCGG-3’ loci
Includes
Epi Mspl: 200 µL
Epi HpaII: 200 µL
10X EpiBuffer: 1 mL
Control pUC19/SmaI DNA Unmethylated (0.5 µg/µL): 20 µL
Control pUC19/SmaI DNA CpG Methylated (0.5 µg/µL): 20 µL
5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.