上海浩洋生物科技有限公司

 EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
  • EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
  • EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

    参考价格: ¥1082.00
    品  牌:MBI
    产品型号:K1441 (200 react)
    所在地区:上海
    上架时间:2013年03月13日
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
    产品名称:EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
    规格:200 react
    货号:K1441
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    10X EpiBuffer Composition 1 mg/mL BSA, 330 mM Tris-acetate (pH 7.9), 1 mM Acetic acid, 660 mM Potassium acetate
    Quality Control Functional Testing
    The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI.
    Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes.
    Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.
    Highlights
    Fast – complete digestion of genomic DNA in 1 hour at 37°C
    Efficient – specially formulated enzymes for epigenetic studies of DNA methylation status
    Applications
    CpG methylation analysis at 5’-CCGG-3’ loci
    Includes
    Epi Mspl: 200 µL
    Epi HpaII: 200 µL
    10X EpiBuffer: 1 mL
    Control pUC19/SmaI DNA Unmethylated (0.5 µg/µL): 20 µL
    Control pUC19/SmaI DNA CpG Methylated (0.5 µg/µL): 20 µL
    5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
    The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.