产品名称：EpiJET DNA Methylation Analysis Kit (MspI/HpaII)
Storage Condition -20 C
10X EpiBuffer Composition 1 mg/mL BSA, 330 mM Tris-acetate (pH 7.9), 1 mM Acetic acid, 660 mM Potassium acetate
Quality Control Functional Testing
The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI.
Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes.
Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.
Fast – complete digestion of genomic DNA in 1 hour at 37°C
Efficient – specially formulated enzymes for epigenetic studies of DNA methylation status
CpG methylation analysis at 5’-CCGG-3’ loci
Epi Mspl: 200 µL
Epi HpaII: 200 µL
10X EpiBuffer: 1 mL
Control pUC19/SmaI DNA Unmethylated (0.5 µg/µL): 20 µL
Control pUC19/SmaI DNA CpG Methylated (0.5 µg/µL): 20 µL
5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.