产品名称：Fast DNA End Repair Kit
Storage Condition -20 C
Quality Control Endo- and Exonucleases
The End Repair Enzyme Mix storage buffer and 10X End Repair Reaction Mix were tested for the absence of contaminating endonucleases and exonucleases using the labeled oligonucleotide test.
Blunting efficiency: 3’- and 5’-overhanging oligoduplexes incubated with 2.5 µL of End Repair Enzyme Mix in 50 µL of 1X End Repair Reaction Mix for 5 min and 20 min at 20°C in the presence of [γ-33P]-ATP, then separated on denaturing PAGE and detected by phosphoimaging, resulted in ≥ 95% of expected bands specific for the blunt end reaction product.
Efficient - blunting and phosphorylation of 0.5 to 5 µg DNA
Fast – reaction is completed in 5 minutes
Convenient – reaction components are premixed to reduce pipetting steps
Blunting and phosphorylation of double-stranded DNA (nebulized DNA, sonicated DNA, restriction enzyme digested DNA, cDNA, PCR products)
The Thermo Scientific Fast DNA End Repair Kit is used for blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation.
All components of the kit contain premixed reagents to reduce pipetting steps and provide convenience. The End Repair Enzyme Mix contains an optimized mixture of T4 DNA Polymerase and Klenow Fragment to achieve highly effective blunting of fragmented DNA, and T4 Polynucleotide Kinase (PKN) for efficient phosphorylation of DNA ends. The 10X End Repair Reaction Mix contains an optimized reaction buffer, ATP, and dNTPs.