上海浩洋生物科技有限公司

 Terminal Deoxynucleotidyl Transferase
  • Terminal Deoxynucleotidyl Transferase
  • Terminal Deoxynucleotidyl Transferase

    参考价格: ¥1607.00
    品  牌:MBI
    产品型号:EP0162 (2500 units)
    所在地区:上海
    上架时间:2013年03月14日
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    详细说明

    Terminal Deoxynucleotidyl Transferase
    产品名称:Terminal Deoxynucleotidyl Transferase
    规格:2500 units
    货号:EP0162
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    5X Reaction Buffer 1 M potassium cacodylate, 125 mM Tris, 0.05% (v/v) Triton X-100, 5 mM CoCl2 (pH 7.2 at 25°C)
    Concentration 20 U/µL
    Definition of Activity Unit
    One unit of the enzyme catalyzes the incorporation of 1 nmol of deoxythymidylate into a polynucleotide fraction (adsorbed on DE-81) in 60 min at 37°C.
    Enzyme activity is assayed in the following mixture: 200 mM potassium cacodylate (pH 7.2), 1 mM CoCl2, 0.01% (v/v) Triton X-100, 10 µM oligo(dT)10, 1 mM dTTP, and 0.4 MBq/mL [3H]-dTTP.
    Inactivation Inactivated by heating at 70°C for 10 min or by addition of EDTA.
    Inhibition Inhibitors: metal chelators, ammonium, chloride, iodide, phosphate ions
    Quality Control The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
    Source E.coli cells carrying a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase.
    Storage Buffer The enzyme is supplied in 100 mM potassium acetate (pH 6.8), 2 mM 2-mercaptoethanol, 0.01% (v/v) Triton X-100 and 50% (v/v) glycerol.
    Highlights
    Incorporates modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)
    Applications
    Production of synthetic homo- and heteropolymers (see? Reference 1)
    Homopolymeric tailing of linear duplex DNA with any type of 3-OH terminus (see? References 2, 3)
    Oligodeoxyribonucleotide and DNA labeling (see? References 2, 4-8)
    5-RACE (Rapid Amplification of cDNA Ends) (see? Reference 9)
    In situ localization of apoptosis (see? Reference 10)