上海浩洋生物科技有限公司

 HEK-Blue mTLR7 cells
  • HEK-Blue mTLR7 cells
  • HEK-Blue mTLR7 cells

    参考价格: ¥15952.00
    品  牌:invivogen
    产品型号:hkb-mtlr7 (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013年03月15日
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    HEK-Blue mTLR7 cells
    产品名称:HEK-Blue mTLR7 cells
    规格:5-7 x 10e6 cells
    货号:hkb-mtlr7
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: blasticidin, Zeocin™
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    HEK-Blue™-mTLR7 cells were obtained by co-transfection of the murine TLR7 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells. The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR7 ligand activates NF-κB and AP-1 which induces the production of SEAP. Levels of SEAP can be easily determined with QUANTI-Blue™ a detection medium that turns purple/blue in the presence of alkaline phosphatase. Alternatively, HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP can be used.
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