Working concentration: 30 µg/ml
Endotoxin level: <0.125 EU/mg
Solubility: 1 mg/ml in culture medium or chloroform
OxPAPC is provided as a solid and shipped at room temperature. Store at -20°C. Solid product is stable 1 year at -20°C. Aliquots of OxPAPC resuspended in chloroform can be stored at -70°C for 3 months. Avoid repeated freeze-thaw cycles.
OxPAPC is generated by the oxidation of 1-palmitoyl-2-arachidonyl-sn- glycero-3-phosphorylcholine (PAPC), which results in a mixture of oxidized phospholipids containing either fragmented or full length oxygenated sn-2 residues. OxPAPC has been shown to inhibit the signaling induced by bacterial lipopeptide and lipopolysaccharide (LPS). It acts by competing with CD14, LBP and MD2, the accessory proteins that interact with bacterial lipids, thus blocking the signaling of TLR2 and TLR4 [1, 2].