Specificity: TLR2 agonist
Working concentration: 106-108 cells/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)
Solubility: Insoluble (resuspended HKMF results in a cloudy suspension)
HKMF is provided lyophilized and shipped at room temperature. Store at 4°C. Upon resuspension, prepare aliquots of HKMF and store at 4°C for short term storage or -20°C for long storage. Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.
Mycoplasma fermentans, a member of the mycoplasma family, is a cell wall-less bacterium. It contains lipopeptides, in particular 2-kDa macrophage-activating lipopetide (MALP-2), a potent stimulator of macrophages through TLR2 and TLR6. Stimulation with heat killed M. fermentans (HKMF) induces rapid activation of NF-κB and the production of pro-inflammatory cytokines.