上海浩洋生物科技有限公司

 pCpG Giant
  • pCpG Giant
  • pCpG Giant

    参考价格: ¥2086.00
    品  牌:invivogen
    产品型号:tlrl-cpgg (1 mg)
    所在地区:上海
    上架时间:2013年03月18日
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    pCpG Giant
    产品名称:pCpG Giant
    规格:1 mg
    货号:tlrl-cpgg
    厂商:invivogen
    产品介绍:
    Activity: 
    CDS agonist
    TLR9 Ligand control
    Formulation: naked or complexed with the transfection reagent LyoVec™
    Working concentrations:
    30 ng - 10 μg/ml transfected pCpGfree-giant
    300 ng - 10 μg/ml pCpGfree-giant/LyoVec™
    Endotoxin level: EndoFit™ (<0.001 EU/µg)
    Solubility:  1 mg/ml in water
    pCpGfree-giant is a high molecular weight plasmid (~15 kb) entirely devoid of CpG dinucleotides and containing AT-rich regions. This DNA also features no detectable amounts of endotoxin, as determined by the LAL assay and the HEK-Blue™-4 cell line-based assay and no detectable TLR2 activity.
    In addition, this plasmid DNA displays no Dcm methylation and a reduced level of Dam methylation. pCpG Giant can be used as a control in studies on CpG methylations.
    Moreover, when transfected in ISG-reporter cells, pCpGfree-giant induces strong reporter activity. This activity requires STING as cells deficient for STING fail to respond to transfected pCpGfree-Giant. The CDSs of pCpGfree-giant have not been identified yet, but they probably include IFI16 and DDX41.