TLR9 Ligand control
Formulation: naked or complexed with the transfection reagent LyoVec™
30 ng - 10 μg/ml transfected pCpGfree-giant
300 ng - 10 μg/ml pCpGfree-giant/LyoVec™
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 1 mg/ml in water
pCpGfree-giant is a high molecular weight plasmid (~15 kb) entirely devoid of CpG dinucleotides and containing AT-rich regions. This DNA also features no detectable amounts of endotoxin, as determined by the LAL assay and the HEK-Blue™-4 cell line-based assay and no detectable TLR2 activity.
In addition, this plasmid DNA displays no Dcm methylation and a reduced level of Dam methylation. pCpG Giant can be used as a control in studies on CpG methylations.
Moreover, when transfected in ISG-reporter cells, pCpGfree-giant induces strong reporter activity. This activity requires STING as cells deficient for STING fail to respond to transfected pCpGfree-Giant. The CDSs of pCpGfree-giant have not been identified yet, but they probably include IFI16 and DDX41.