pDUO plasmid with human MD2 and human TLR4 isoform A genes.
Name: MD2 / TLR4A
pDUO plasmid with murine MD2 and murine TLR4 genes.
Name: MD2 / TLR4
pDUO-MD2/TLR4 is an expression vector designed to co-express MD2 and TLR4 genes known to interact with each other : human MD2 (480 bp) and human TLR4A (2517 bp) or murine MD2 (480 bp) and murine TLR4 (2505 bp).
The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.
TLR4 is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to LPS.
However, TLR4 alone is not sufficient to confer LPS responsiveness. TLR4 requires MD-2, a secreted molecule, to functionaly interact with LPS [1,2]. TLR4 physically associates with MD2, and together with a third protein called CD14, this complex is responsible for LPS recognition and signaling .