上海浩洋生物科技有限公司

 ReadiView Biotin hydrazide
  • ReadiView Biotin hydrazide
  • ReadiView Biotin hydrazide

    参考价格: ¥2340.00
    品  牌:AAT Bioquest
    产品型号:AAT-3055 (5 mg)
    所在地区:上海
    上架时间:2013年04月10日
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    ReadiView Biotin hydrazide
    产品名称:ReadiView Biotin hydrazide
    产品货号:AAT-3055
    产品价格:¥2340
    产品规格:5 mg
    MW:552.60          Solvent:DMSO
    Storage:F/D (see storage codes below)
    Biotin/avidin complexes are widely applied for a variety of biological detections. Although a large number of biotin-labeled bioconjugates are commercially available, the accurate determination of biotinylation degree (ratio of biotin/biopolymer) is still a great challenge for biochemists. HABA is still predominantly used for determining the degree of biotinylation (through its absorption with the extinction coefficient = 34,000/M-1cm-1). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA. The resulting decrease in absorbance relates to the amount of biotin. However there are many factors that affect the accuracy of HABA method, making this method unreliable for many biotin-labeled conjugates. Our ReadiView?biotin contains specially designed Color Tag (CT) that makes the biotinylation degree readily accessible by simply calculating the corrected absorption ratio of 280 nm/385 nm. Our specially designed tag has very minimal effect on the biotin binding affinity, and its absorption maximum was designed to make the tag have minimal quenching effect on the most fluorophores that are used for labeling avidins.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15 )
    R = Refrigerated (2-4 )
    RT = Room temperature (4-50 )