上海浩洋生物科技有限公司

 DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]
  • DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]
  • DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]

    参考价格: ¥1140.00
    品  牌:AAT Bioquest
    产品型号:AAT-17513 (25 mg)
    所在地区:上海
    上架时间:2013年04月11日
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    DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]
    产品名称:DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]
    产品货号:AAT-17513
    产品价格:¥1140
    产品规格:25 mg
    Ex (nm):358              Em (nm):461
    MW:350.25                Solvent:Water
    Storage:F/D/L (see storage codes below)
    DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPIs blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15 )
    R = Refrigerated (2-4)
    RT = Room temperature (4-50)