上海浩洋生物科技有限公司

 Indo-1 AM UltraPure grade
  • Indo-1 AM UltraPure grade
  • Indo-1 AM UltraPure grade

    参考价格: ¥2100.00
    品  牌:AAT Bioquest
    产品型号:AAT-21036 (20x50 ug)
    所在地区:上海
    上架时间:2013年04月11日
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    Indo-1 AM UltraPure grade
    产品名称:Indo-1 AM UltraPure grade
    产品货号:AAT-21036
    产品价格:¥2100
    产品规格:20x50 ug
    Ex (nm):346              Em (nm):475 
    MW:1009.91               Solvent:DMSO 
    Storage:F/D/L (see storage codes below)
    Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fura-2 and Indo-1 are most commonly used among the UV-excitable calcium indicators. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. In contrast, Indo-1 is the preferred dye for flow cytometry, where it is more practical to use a single laser for excitation (usually the 351?64 nm spectral lines of the argon-ion laser). The emission of Indo-1 shifts to 400 nm (when bound to Ca2+) from 480 nm in Ca2+-free environments. Indo-1 AM is cell-permeable version of Indo-1.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15)
    R = Refrigerated (2-4)
    RT = Room temperature (4-50)