Coelenterazine n UltraPure grade_中国教育装备采购网


 Coelenterazine n UltraPure grade
  • Coelenterazine n UltraPure grade
  • Coelenterazine n UltraPure grade

    参考价格: ¥1140.00
    品  牌:AAT Bioquest
    产品型号:AAT-21155 (250 ug)

    Coelenterazine n UltraPure grade
    产品名称:Coelenterazine n UltraPure grade
    产品规格:250 ug
    Ex (nm):431                 Em (nm):468
    MW:457.52                   Solvent:Ethanol
    Storage:F/D/L (see storage codes below)
    Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorins bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1  to >100 . Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10 0 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15)
    R = Refrigerated (2-4)
    RT = Room temperature (4-50)