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 CFDA [5-(and 6)-Carboxyfluorescein diacetate] Mixed isomers
  • CFDA [5-(and 6)-Carboxyfluorescein diacetate] Mixed isomers
  • CFDA [5-(and 6)-Carboxyfluorescein diacetate] Mixed isomers

    参考价格: ¥900.00
    品  牌:AAT Bioquest
    产品型号:AAT-22021 (100 mg)
    所在地区:上海
    上架时间:2013年04月12日
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    CFDA [5-(and 6)-Carboxyfluorescein diacetate] Mixed isomers
    产品名称:CFDA [5-(and 6)-Carboxyfluorescein diacetate] Mixed isomers
    产品货号:AAT-22021
    产品价格:¥900
    产品规格:100 mg
    Ex (nm):494                     Em (nm):521
    MW:460.39                       Solvent:DMSO            
    Storage:F/D/L (see storage codes below)
    It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. CFDA is an amine eactive FDA derivative that can be used to prepare a variety of FDA conjugates.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15)
    R = Refrigerated (2-4)
    RT = Room temperature (4-50)