上海浩洋生物科技有限公司

 CytoTell Red
  • CytoTell Red
  • CytoTell Red

    参考价格: ¥1740.00
    品  牌:AAT Bioquest
    产品型号:AAT-22256 (1000 Tests)
    所在地区:上海
    上架时间:2013年04月12日
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    CytoTell Red
    产品名称:CytoTell Red
    产品货号:AAT-22256
    产品价格:¥1740
    产品规格:1000 Tests
    Ex (nm):628                   Em (nm):643
    MW:~600                       Solvent:DMSO
    Storage:F/D/L (see storage codes below)
    Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell?dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions. CytoTell?dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell?Red is a red fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Up to 8 generations may be visualized. CytoTell?Red can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation. Cells labeled with Cell CytoTell?Red may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell?Red has a peak excitation of 630 nm and can be excited by the red (633 nm) laser line. It has a peak emission of 660 nm and can be detected with a 660/20 band pass filter (equivalent to APC, Alexa Fluor 647, or Cy5, making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis.
    Storage Codes:
    D = Desiccated
    L = Avoid light
    F = Freeze (<-15)
    R = Refrigerated (2-4)
    RT = Room temperature (4-50)