上海浩洋生物科技有限公司

Screen Quest Luminometric Calcium Assay Kit

Screen Quest Luminometric Calcium Assay Kit

参考价格:¥3540.00
品  牌:AAT Bioquest
产品型号:AAT-36305 (10 Plates)
所在地区:上海
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Screen Quest Luminometric Calcium Assay Kit
产品名称:Screen Quest Luminometric Calcium Assay Kit
产品货号:AAT-36305
产品价格:¥3540
产品规格:10 Plates
Ex (nm):437                Em (nm):466
Storage:F/D/L (see storage codes below)
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine ased kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 )
R = Refrigerated (2-4 )
RT = Room temperature (4-50 )