上海浩洋生物科技有限公司

Screen Quest Fluo-8 Medium Removal Calcium Assay Kit Optimized for Difficult Cell Lines

Screen Quest Fluo-8 Medium Removal Calcium Assay Kit Optimized for Difficult Cell Lines

参考价格:¥2940.00
品  牌:AAT Bioquest
产品型号:AAT-36308 (10 Plates)
所在地区:上海
留言咨询
与企业取得联系时请告知该信息获取自中国教育装备采购网
我要分享
详细说明

Screen Quest Fluo-8 Medium Removal Calcium Assay Kit Optimized for Difficult Cell Lines
产品名称:Screen Quest Fluo-8 Medium Removal Calcium Assay Kit Optimized for Difficult Cell Lines
产品货号:AAT-36308
产品价格:¥2940
产品规格:10 Plates
Ex (nm):490               Em (nm):525
Storage:F/D/L (see storage codes below)
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest?Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 NW are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW. The characteristics of its long wavelength, high sensitivity, and 100-250 times fluorescence increases (when it forms complexes with calcium) make Fluo-8 NW an ideal indicator for measurement of cellular calcium. This Screen Quest Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 )
R = Refrigerated (2-4 )
RT = Room temperature (4-50 )