上海浩洋生物科技有限公司

Screen Quest Fluorimetric MDR Assay Kit

Screen Quest Fluorimetric MDR Assay Kit

参考价格:¥2340.00
品  牌:AAT Bioquest
产品型号:AAT-36340 (1 kit)
所在地区:华东 上海
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Screen Quest Fluorimetric MDR Assay Kit
产品名称:Screen Quest Fluorimetric MDR Assay Kit
产品货号:AAT-36340
产品价格:¥2340
产品规格:1 kit
Ex (nm):492              Em (nm):514
Storage:F/D/L (see storage codes below)
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Some tumors are initially resistant and never respond to cytostatic drug treatment; others initially respond well but eventually regrow and become resistant. This phenomenon may result from genetic mutations induced by the administered antitumor agent, or may represent the selection of preexisting resistant cell populations in the malignant tumor. Multi-drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP-dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug-resistance-associated protein (MRP1) are members of the ABC transporter family. Our assay kit uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporter, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Our MDR assay kit provides all the essential components with an optimized assay method. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. This assay kit is ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15)
R = Refrigerated (2-4)
RT = Room temperature (4-50)