上海浩洋生物科技有限公司

Screen Quest Colorimetric ELISA cAMP Assay Kit

Screen Quest Colorimetric ELISA cAMP Assay Kit

参考价格:¥3540.00
品  牌:AAT Bioquest
产品型号:AAT-36370 (1 plate)
所在地区:华东 上海
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Screen Quest Colorimetric ELISA cAMP Assay Kit
产品名称:Screen Quest Colorimetric ELISA cAMP Assay Kit
产品货号:AAT-36370
产品价格:¥3540
产品规格:1 plate
Ex (nm):650                   
Storage:F/D/L (see storage codes below)
Adenosine 3  5 cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring levels of cAMP is one of the most common ways to screen for agonists and antagonists of GPCRs. Screen Quest Colorimetric ELISA cAMP Assay Kit is based on the competition between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest?Colorimetric ELISA cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite?Blue as a colorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15)
R = Refrigerated (2-4)
RT = Room temperature (4-50)