产品名称：HiLite Histone H3 Methyl-Lys9 / Lys27 Binding Assay
HiLite Histone Binding Assay advantages
•All the reagents and a detailed protocol have been developed to help you perform methyl-histone binding assays to examine proteins that bind to either methyl Lys9 or methyl-Lys27 of histone H3.
•FP is a homogeneous technology with no washing steps. This increases speed and precision.
•Reactions are very rapid, generally taking only seconds to minutes to reach equilibrium.
•FP experiments are non-perturbing, so the influence on binding of multiple factors can be analyzed on the same samples (e.g. changes in pH, temperature, salt concentration, etc.).
Contents & Storage
The HiLite™ Histone H3 Methyl-Lys9 / Lys27 Binding Assay includes eight fluorescently conjugated histone peptides (unmodified H3 Lys9, H3 monomethyl Lys9, H3 dimethyl Lys9, H3 trimethyl Lys9, unmodified H3 Lys27, H3 monomethyl Lys27, H3 dimethyl Lys27 and H3 trimethyl Lys27), Binding Buffer, Calibration dye, HP1 positive control protein, DTT and five black half area 96-well plates. Storage conditions vary from room temperature to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.