产品名称：Histone H3K79me2 antibody (pAb)
This Histone H3 dimethyl Lys79 antibody (H3K79me2) has been validated for use in chromatin IP (10 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:500 - 1:5,000). Specificity for dimethyl lysine 79 was confirmed by dot blot with non-modified histone H3 peptide or peptides mono-, di- or trimethylated at lysine 79. In addition, Western blot against 200 ng of recombinant histone H3 was negative. This product is specific for histone H3 dimethylated at lysine 79. Because this modification is conserved from yeast to human, a wide range of reactivity is expected.
The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 dimethyl Lys79 antibody was validated for ChIP-Seq in the study (see References).
This Histone H3 dimethyl Lys79 antibody was raised against a peptide including dimethyl-lysine 79 of histone H3.
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39923) of this antibody that was purified by Protein A Chromatography is also available.
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.