上海浩洋生物科技有限公司

 Hydroxymethyl Collector
  • Hydroxymethyl Collector
  • Hydroxymethyl Collector

    参考价格: ¥5530.00
    品  牌:Active Motif
    产品型号:55013 (25 rxns)
    所在地区:上海
    上架时间:2013年04月16日
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    Hydroxymethyl Collector
    产品名称:Hydroxymethyl Collector
    产品货号:55013
    产品价格:¥5530
    产品规格:25 rxns
    Contents & Storage
    Hydroxymethyl Collector™ contains all thre reagents needed to perform the glucosylation reaction, biotin conjugation, streptavidin capture, elution and purification of DNA containing 5-hydroxymethylcytosine. Protocols are provided for the fragmentation of sample DNA all the way through to real time PCR analysis of a locus of interest. For added convenience, the kit also includes positive control 5-hmC DNA, which is a 338 bp sequence of the APC locus in which 25% of the cytosine residues have been hydroxymethylated. PCR primers for APC are provided to confirm the efficiency of the enrichment reactions.
    Each kit contains β-glucosyltransferase enzyme, UDP-Azide-Glucose, 10X Reaction Buffer AM1, 1 M DTT, Biotin Conjugate Solution, Streptavidin Beads, Binding Buffer AM13, 10X Ellution Buffer AM2, DNA Purification Binding Buffer, DNA Purification Wash Buffer, DNA Purification Elution Buffer, 3 M Sodium Acetate, DNA purification columns, 5-hmC Control DNA, APC PCR primer mix, 0.2 ml PCR tubes, a bar magnet and glue dots.
    Storage conditions range from room temperature to -20°C; please refer to the product manual for proper storage temperatures of each component.