Contents & Storage
Hydroxymethyl Collector™ contains all thre reagents needed to perform the glucosylation reaction, biotin conjugation, streptavidin capture, elution and purification of DNA containing 5-hydroxymethylcytosine. Protocols are provided for the fragmentation of sample DNA all the way through to real time PCR analysis of a locus of interest. For added convenience, the kit also includes positive control 5-hmC DNA, which is a 338 bp sequence of the APC locus in which 25% of the cytosine residues have been hydroxymethylated. PCR primers for APC are provided to confirm the efficiency of the enrichment reactions.
Each kit contains β-glucosyltransferase enzyme, UDP-Azide-Glucose, 10X Reaction Buffer AM1, 1 M DTT, Biotin Conjugate Solution, Streptavidin Beads, Binding Buffer AM13, 10X Ellution Buffer AM2, DNA Purification Binding Buffer, DNA Purification Wash Buffer, DNA Purification Elution Buffer, 3 M Sodium Acetate, DNA purification columns, 5-hmC Control DNA, APC PCR primer mix, 0.2 ml PCR tubes, a bar magnet and glue dots.
Storage conditions range from room temperature to -20°C; please refer to the product manual for proper storage temperatures of each component.