氧化型低密度脂蛋白(Human Ox-LDL, OxLDL)
|CAS号：||MF, AF, Tbars, (>2mg/ml) C 98% 2mg|
OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN
Catalog No: AY-1501 Lot No: date
Quantity: 2.0 mg (milligrams) Protein/vial Concentration: lot specific mg/ml (Protein)
Packaging: Oxidized LDL is membrane filtered and aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 5uM EDTA-.NA2.
Preparation: AY Human LDL (Cat. No. AY-1502) (which was purified to homogeneity via ultra-centrifugation(1.019-1.063g/cc)) is oxidized using 5uM Cu2SO4 (oxidant) in PBS at 37°C for 20 hours. Oxidation is terminated by adding excess EDTA-.NA2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.
TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard.
Starting LDL lot specific nmoles of MDA/mg Protein
Ox-LDL lot specific nmoles of MDA/mg Protein
Stability: This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C. NEVER FREEZE.
Special Note: After prolonged storage,some precipitate may be observed. This is normal for this product.Clarify out the aggregates by spinning in a microfuge for 2 minutes.
Biological Sample lots of AY Oxidized LDL are evaluated for receptor binding to
Activity: peritoneal macrophages in conjunction with our DiI-Ox-LDL and [I-125] Ox-LDL
*Preparations of Oxidized LDL are fairly unstable; plan your experiments in advance and use fresh material.
FOR RESEARCH USE ONLY