PageRuler™ Prestained Protein Ladders are designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency and approximate sizing of proteins (1-3). The PageRuler™ Prestained Protein Ladder is a mixture of 10 recombinant, highly purified, colored proteins with apparent molecular weights of 10 to 170 kDa. Ladder proteins are covalently coupled with a blue dye except for two reference bands prestained with different colors. The 72kDa reference band is orange and 10kDa reference band is green.
PageRuler™ Prestained Protein Ladders can be used in Western blotting on PVDF (see protocol for Western Blotting, nylon and nitrocellulose membranes).