Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
? ELISA: 50 to 150 mU/ml
? Immunohistocytochemistry: 250 to 500 mU/ml
? In situ hybridization: 1.5 to 7.5 U/ml
? Southern blot: 150 mU/ml
? Western blot: 250 to 500 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.