北京冬璞泰和科技有限责任公司

第9年
4006816680转180
DIG RNA Labeling Mix

DIG RNA Labeling Mix

参考价格:¥2083.00
品  牌:ROCHE
产品型号:11277073910
适用范围:高教
所在地区:尚未填写城市
上架时间:2017-11-30
留言咨询
4006816680-180  QQ交谈
与企业取得联系时请告知该信息获取自中国教育装备采购网
我要分享
联系我们
$contactus$
热门产品
产品分类
  •   常用生化试剂
  • 核酸电泳试剂类
  • 细菌培养接种
  • 厌氧耗材类
  • 西班牙DEL
  • 加拿大Labplas
  •   美国Pall
  • 英国Whatman
  • 美国Wheaton
  • Merck-Millipore
  • 美国VWR
  • 美国ABI
  • 德国Brand
  • 美国Nalgene
  • 德国QIAGEN
  • 美国BD
  • 丹麦Nunc
  • 美国Axygen
  • 德国Greiner
  • 美国Corning 康宁
  • 详细说明
    DIG RNA Labeling Mix
    Labeling efficiency: Approximately 10μg of full-length digoxigenin-labeled RNA is transcribed from 1μg linear template DNA.
    Assay Time: 135 minutes
    Sample Materials
    Linearized plasmid DNA:
    The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of ‘run off’ transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5′-overhangs should be used; 3′ overhangs should be avoided. The linearized template DNA should be purified by phenol chloroform extraction and ethanol precipitation, to avoid RNase contamination. For ′run around′ transcription circular plasmid DNA is used.
    PCR product:
    PCR-fragments which contain RNA polymerase promoter sequences can also be used as templates for transcription. Purification of the PCR fragment by HighPure column purification prior to transcription is recommended.

    DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. DIG-11-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The DIG RNA Labeling Mix is specifically designed for the use with SP6, T7, and T3 RNA polymerases, which are supplied with an optimized transcription buffer.
    Convenient nucleotide mixture for the labeling of RNA with Digoxigenin-11-UTP.

    Contents
    10x solution with:
    10 mM ATP, CTP, GTP (each), 6.5 mM UTP, 3.5 mM DIG-11-UTP.

    Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.