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 Merck  rLysozyme? Solution71110-3 71110-4 71110-5
  • Merck  rLysozyme? Solution71110-3 71110-4 71110-5
  • Merck rLysozyme? Solution71110-3 71110-4 71110-5

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    品  牌:Merck
    产品型号:71110-3 71110-4 71110-5 (71110-3 71110-4 7111)
    所在地区:
    上架时间:2018年10月15日
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  • 详细说明

     Merck  rLysozyme? Solution71110-3 71110-4 71110-5

    rLysozyme? Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/μl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.

    产品信息

    Avoid freeze/thaw

    是的

    Unit definition

    One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner?(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.

    运输信息

    Storage

    -20°C

    Ship

    Blue Ice Only 
    Standard Handling