New features of this version are:
Calculation of Tm now including "dangling end"parameters (if applicable).
Maximum possible sequence length increased from 256bp to 1755bp for all features.
Maximum possible alignment for cross hybridization is shown graphically.
Mutations can be inserted by bp and base exchange.
A formula for calculation of Tm for long sequences is added .
Dangling end calculations are included into the algorithms.
(Highly important for optimization of hyb-probes; every probe has two dangling ends, since the DNA template is usually longer than the probes!).
Space between anchor and probes for LightCycler™ assays can be chosen (1 to 5). Minor modifications:
Mismatches are highlighted automatically.
Calculation of sodium equivalents has been modified.
SYBR green-I™ influence on Tm is estimated, based on empirical data.