上海经科化学科技有限公司

 pRNAin-H1.2/Neo
  • pRNAin-H1.2/Neo
  • pRNAin-H1.2/Neo

    参考价格: ¥0.00
    适用范围:全领域
    所在地区:
    上架时间:2011年08月21日
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    详细说明

    pRNAin-H1.2/Neo is a Genscript inducible siRNA expression vector. The H1.2 promoter is an inducible promoter* containing a tetracycline operator (TetO1). The tetracycline operator itself has no effect on expression.

    Description: pRNAin-H1.2/Neo is a Genscript inducible siRNA expression vector. The H1.2 promoter is an inducible promoter* containing a tetracycline operator (TetO1). The tetracycline operator itself has no effect on expression. When the tetracycline repressor (TetR) is present, it effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR and causes the TetR protein to release the TetO1 site, derepressing the transcription from the H1 promoter.

    pRNAin-H1.2/Neo

    pRNAin-H1.2/Neo is designed for mammalian transfection. It carries a neomycin resistance gene that can be used for establishing stable cell line.

    pRNAin-H1.2/Neo
    pRNAin-H1.2/Neo
    Download Protocol:tm0169
    Forward Sequencing Primer:
    DA0013:pRNA-H1 Forward (TAATACGACTCACTATAGGG)
    Reverse Sequencing Primer:
    DA0012:pRNA Reverse (TAGAAGGCACAGTCGAGG)
    Detailed Map:
    Polylinker:4700-4770
    H1 Promoter:4600-4699
    SV40:647-992
    Neomycin:1033-1827
    pUC ori:2541-3181
    Ampicillin:3329-4189
    Publications used this GenScript Product:
  • Qi Cai, Natalia I. Dmitrieva, Joan D. Ferraris, Heddwen L. Brooks, Bas W. M. van Balkom, and Maurice Burg. Pax2 expression occurs in renal medullary epithelial cells in vivo and in cell culture, is osmoregulated, and promotes osmotic tolerance. Proc. Natl. Acad. Sci. USA. Jan 2004; 102 (2) :503-508
  • Burnett BG, Pittman RN. The polyglutamine neurodegenerative protein ataxin 3 regulates aggresome formation. Proc. Natl. Acad. Sci. USA. Mar 2005; 102 (12) :4330-4335
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