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 Uracil DNA Glycosylase
  • Uracil DNA Glycosylase
  • Uracil DNA Glycosylase

    参考价格: ¥0.0000
    适用范围:全领域
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    上架时间:2011/8/21 0:00:00
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    详细说明

    Description:E. coli Uracil DNA Glycosylase (UNG) catalyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).

    Description:E. coli Uracil DNA Glycosylase (UNG) catalyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).

    Source:An E. coli strain that carries the UNG gene from E. coli.

    Applications:

    • Glycosylase mediated single nucleotide polymorphism detection (GMPD)
    • Site-directed mutagenesis
    • As a probe for protein-DNA interaction studies
    • Rapid and efficientcloning of PCR products
    • Elimination carry-over contamination in PCR

    Quality Control:Activity, SDS-PAGE (purity), 16-hour incubation, exonuclease and endonuclease activity

    Unit Definition:One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 μl in 30 minutes at 37℃ in 1X Uracil DNA Glycosylase Reaction Buffer with 1 unit or uracil DNA Glycosylase and 0.2 μg [3H]-uracil DNA (104-105 cpm/μg).

    10X UNG Reaction Buffer:200 mM Tris-HCl (pH8.0 at 25℃), 10 mM Dithiothreitol, 10 mM EDTA.

    Reaction Conditions:1X UNG Reaction Buffer, incubate at 37℃.

    Inhibition and Inactivation:Inactivated by heating at 95℃ for 10 min. Enzyme activity is partially restored at temperatures lower than 55℃.

    Storage Buffer and Concentration:UNG in 10 mM Tris-HCl (pH7.4 at 25℃), 50 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol

    Storage:Store at -20℃.

    Note:UNG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (>200 mM).
    The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.