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 Taq DNA Polymerase, concentrated
  • Taq DNA Polymerase, concentrated
  • Taq DNA Polymerase, concentrated

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    适用范围:全领域
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    上架时间:2011年08月21日
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    详细说明

    Description: Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.

    Description: Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

    Applications: The applications of Taq DNA polymerase are as follows:

    • PCR*
    • 3A-tailing of blunt ends
    • Primer extension
    • DNA sequencing

    Formulation: Genscript Taq DNA Polymerase has been formulated using GenScripts proprietary technology. The enzyme can be shipped at room temperature or stored at 37 ℃ for seven days without any significant loss of activity.

    Features:

    • Terminal transferase activity: A single nucleotide (adenosine) is added to the 3 end of the extension product.
    • High-purity: No contamination activity has been detected in standard test reactions.

    QC Tests: PCR* performance, activity, nuclease


    Taq DNA Polymerase, concentrated
    Lane Taq Amount Used
    1 Leading
    Brand A
    0.1
    2 0.25
    3 0.5
    4 Leading
    Brand B
    0.1
    5 0.25
    6 0.5
    7 GenScript 0.1
    8 0.25
    9 0.5

    Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74 ℃.

    10 X Reaction Buffer (with Mg2+):

    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25℃), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 μM dNTPs.
    Note: 0.1% Triton X-100 (final concentration) is a must to ensure high activity. GenScript Triton X-100 Buffer is provided ready-to-use, but GenScript Taq DNA Polymerase is compatible with other buffers.

    Storage Buffer and Concentration: Supplied in 25 units/μl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

    Storage: Store the product at -20 ℃.