上海经科化学科技有限公司

 High-Stability PCR Kit
  • High-Stability PCR Kit
  • High-Stability PCR Kit

    参考价格: ¥0.00
    适用范围:全领域
    所在地区:
    上架时间:2011年08月21日
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    详细说明

    GenScript High-Stability PCR Kit contains Taq DNA polymerase, 10X buffer, and 10 mM dNTP, all specially formulated for increased stability using our proprietary technology.

    Kit Description:

    GenScript High-Stability PCR Kit contains Taq DNA polymerase, 10X buffer, and 10 mM dNTP, all specially formulated for increased stability using our proprietary technology. The kit can be stored at 4°C for over six months or at room temperature (25°C) for one month without significant loss of activity. The stabilizers in the buffers also increase the stability of Taq DNA polymerase at higher temperature (72°C), increasing the DNA yield of PCR amplifications of long DNA. This kit facilitates quick and easy PCR setup without any need for thawing. This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time.

    Key features:
    • High Stability: The kit remains stable for more than six months when stored at 4°C or for one month when stored at room temperature (25°C) without significant activity loss.
    • Terminal Transferase Activity: Taq DNA polymerase has terminal transferase activity that results in the addition of a single nucleotide (adenosine) at 3′-end of the extension product.
    • Increased PCR Yield: The green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR yield when amplifying long DNA.


    Examples:

  • The green Taq DNA polymerase increases PCR yield when amplifying long DNA.
    High-Stability PCR Kit

    Figure 1. A 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (in this case, the elongation time was eight minutes), the green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR DNA yield significantly.
  • The green Taq DNA polymerase can be stored at 37°C for 14 days without loss of activity.
    High-Stability PCR Kit

    Figure 2. A 2-kb 23S rRNA fragment from E. coli genomic DNA was amplified using green Taq DNA polymerase stored at -20°C and 37°C, respectively. The green Taq DNA polymerase can be stored at 37°C for 14 days without significant activity loss.
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