Medium & Serum:
Mus musculus (mouse)
|Source: || |
Tissue: skeletal muscle
Cell type: myoblast; myoblast
|Permits/Forms: || |
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|Related Cell Culture Products |
|Strain: || |
|Age: || |
|Comments: || |
Sol8 is a myogenic cell line isolated by Daubas et al. from primary cultures of soleus muscle taken from the leg of a normal C3H mouse.
The cells were split two times and then cloned by limiting dilution.
Sol8 cells will differentiate into myotubes with serum deprivation.
They have a phenotype similar to slow twitch fibers.
Note: do not allow the cells to become confluent.
The myoblast component of the population will rapidly become depleted if the culture becomes confluent.
|Propagation: || |
ATCC complete growth medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%; fetal bovine serum, 20%
|Subculturing: || |
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture at 50% of confluence.
Subcultivation ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium renewal: 2 to 3 times per week
|Preservation: || |
Culture medium, 95%; DMSO, 5%
|Doubling Time: || |
16 to 18 hrs
|References: || |
23248: Daubas P , et al. Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems. Nucleic Acids Res. 16: 1251-1271, 1988. PubMed: 2894633