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 Sol8(小鼠骨骼肌肌肉母细胞)
  • Sol8(小鼠骨骼肌肌肉母细胞)
  • Sol8(小鼠骨骼肌肌肉母细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CRL-2174 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    Sol8(小鼠骨骼肌肌肉母细胞)

    Sol8(小鼠骨骼肌肌肉母细胞)
     
    Cell Biology
    ATCC® Number: CRL-2174™ Price: $330.00
    Designations:
    Sol8
    Depositors:
    R Bassel-Duby
    Biosafety Level:
    1
    Shipped:
    frozen
    Medium & Serum:
    See Propagation
    Growth Properties:
    adherent
    Organism:
    Mus musculus (mouse)
    Morphology:
    fibroblast
     
    Source:
    Tissue: skeletal muscle
    Cell type: myoblast; myoblast
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Strain:
    C3H
    Age:
    4 weeks
    Comments:
    Sol8 is a myogenic cell line isolated by Daubas et al. from primary cultures of soleus muscle taken from the leg of a normal C3H mouse.
    The cells were split two times and then cloned by limiting dilution.
    Sol8 cells will differentiate into myotubes with serum deprivation.
    They have a phenotype similar to slow twitch fibers.
    Note: do not allow the cells to become confluent.
    The myoblast component of the population will rapidly become depleted if the culture becomes confluent.
    Propagation:
    ATCC complete growth medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%; fetal bovine serum, 20%
    Subculturing:
    Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
    Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
    Add fresh medium, aspirate and dispense into new flasks.
    Subculture at 50% of confluence.
    Subcultivation ratio: A subcultivation ratio of 1:8 to 1:12 is recommended

    Medium renewal: 2 to 3 times per week
    Preservation:
    Culture medium, 95%; DMSO, 5%
    Doubling Time:
    16 to 18 hrs
    References:
    23248: Daubas P , et al. Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems. Nucleic Acids Res. 16: 1251-1271, 1988. PubMed: 2894633