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 MDBK (NBL-1)(牛肾细胞)
  • MDBK (NBL-1)(牛肾细胞)
  • MDBK (NBL-1)(牛肾细胞)

    参考价格: ¥0.00
    品  牌:ATCC
    产品型号:CCL-22 (株)
    所在地区:上海
    上架时间:2012年08月01日
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    MDBK (NBL-1)(牛肾细胞)

    MDBK (NBL-1)(牛肾细胞)
     
    Cell Biology
    ATCC® Number: CCL-22™ Price: $203.00
    Designations:
    MDBK (NBL-1)
    Depositors:
    S Madin
    NB Darby
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Bos taurus (cow)
    Morphology:
    epithelial
     
    Source:
    Organ: kidney
    Disease: normal
    Cellular Products:
    keratin
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    detection and quantitation of replication competent retroviruses [92434]
    transfection host (technology from amaxa)
    Virus
    Susceptibility:
    vesicular stomatitis (Indiana); infectious bovine rhinotracheitis virus; bovine parvovirus; bovine adenovirus I, III; bovine viral diarrhea viruses; parainfluenzavirus 3
    Virus Resistance:
    poliovirus 2
    Reverse Transcript:
    negative
    Cytogenetic
    Analysis:
    Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%.
    There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DMs were seen.
    Age:
    adult
    Gender:
    male
    Comments:
    The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby.
    It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV).
    In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection.
    The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK.
    The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.
    The cells are positive for keratin by immunofluorescence.
    The line has been shown to be free of bovine viral diarrhea virus (BVDV). [22883]
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.
    Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

    Medium renewal: Twice per week
    Preservation:
    culture medium 95%; DMSO, 5%
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
    References:
    22217: Madin SH , Darby NB Jr. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776
    22883: Bolin SR , et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
    28301: Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
    32728: Russell DW , Miller AD . Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528
    92434: USEPA Manual of Methods for Virology - EPA publication. Washington, DC: Environmental Protection Agency; EPA EPA 600/4-84/013 (R9), 2001.