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 A-375 [A375](恶性黑色素瘤细胞)
  • A-375 [A375](恶性黑色素瘤细胞)
  • A-375 [A375](恶性黑色素瘤细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CRL-1619 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    A-375 [A375](恶性黑色素瘤细胞)

    A-375 [A375](恶性黑色素瘤细胞)
     
    Cell Biology
    ATCC® Number: CRL-1619™ Price: $203.00
    Designations:
    A-375 [A375]
    Depositors:
    DJ Giard
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Homo sapiens (human)
    Morphology:
    epithelial
    A-375 [A375](恶性黑色素瘤细胞)
     
    Source:
    Organ: skin
    Disease: malignant melanoma
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    Tumorigenic:
    Yes, in immunosuppressed mice
    DNA Profile
    (STR):
    Amelogenin: X
    CSF1PO: 11,12
    D13S317: 11,14
    D16S539: 9
    D5S818: 12
    D7S820: 9
    THO1: 8
    TPOX: 8,10
    vWA: 16,17
    Cytogenetic
    Analysis:
    It is a hypotriploid with a modal number of 62 chromosomes. There are 9 marker chromosomes that are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.
    Age:
    54 years
    Gender:
    female
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.


        Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

        Medium renewal: Every 2 to 3 days
    Preservation:
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
    recommended serum: ATCC 30-2020
    derivative: ATCC CRL-1872
    References:
    23218: Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
    23221: Gershwin ME , et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033
    32493: Cormier JN , et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int. J. Cancer 75: 517-524, 1998. PubMed: 9466650
    32832: Sharma SD , et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc. Natl. Acad. Sci. USA 93: 13715-13720, 1996. PubMed: 8943000