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 5637(膀胱癌细胞)
  • 5637(膀胱癌细胞)
  • 5637(膀胱癌细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:HTB-9 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    5637(膀胱癌细胞)

    5637(膀胱癌细胞)
     
    Cell Biology
    ATCC® Number: HTB-9™ Price: $255.00
    Designations:
    5637
    Depositors:
    G Cannon
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Homo sapiens (human)
    Morphology:
    epithelial
     
    Source:
    Organ: urinary bladder
    Disease: carcinoma
    Tumor stage: grade II
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation:
    Isolation date: March, 1974
    Applications:
    Tumorigenic:
    Yes, within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
    DNA Profile
    (STR):
    Amelogenin: X,Y
    CSF1PO: 11
    D13S317: 11
    D16S539: 9
    D5S818: 11,12
    D7S820: 10,11
    THO1: 7,9
    TPOX: 8,9
    vWA: 16,18
    Cytogenetic
    Analysis:
    modal chromosome number = 67; range = 59 to 71 The stemline modal chromosome number is 67 occurring at 36%, and polyploidy at 0.6%. Fourteen marker chromosomes were common to these cells: 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q) and 8 others of unknown origin. Additional markers: der(5)t(5;7)(q31;p11) and 1p were found that were specific to a minor subpopulation. Microchromosomes (larger than double minutes) and double minutes (DM) were also found in a small population of cells. Normal N13 was absent; and N21 was found only in the minor subpopulation.One, or occasionally two, Y chromosomes were present in each cell.
    Isoenzymes:
    AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 2
    Age:
    68 years adult
    Gender:
    male
    Ethnicity:
    Caucasian
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

    Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

    Medium renewal: 2 to 3 times per week
    Preservation:
    Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
    recommended serum: ATCC 30-2020
    parental strain: ATCC CRL-2742
    References:
    22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
    24381: Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
    25065: Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
    32266: Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064
    32283: Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
    32773: Schnier JB , et al. G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637. Proc. Natl. Acad. Sci. USA 93: 5941-5946, 1996. PubMed: 8650198